EN 1650:2008+A1:2013

Quantitative suspension test for the evaluation of fungicidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic, and institutional areas.
II Phase / I step

Test organisms:
Aspergillus brasiliensis (previously A. niger) ATCC 16404
Candida albicans ATCC 10231

Temperature (°C): 20
Contact time (min.): 15
*or client requirements

Interfering substances:
Clean conditions – bovine albumin 0,3 g/l
Dirty conditions – bovine albumin 3,0 g/l

Reduction (lg): ≥ 4,0
The following additional test conditions are permitted:
Saccharomyces cerevisiae ATCC 9763
Saccharomyces cerevisiae DSM 70487
var. diastaticus
Additional strains are permitted

Temperature (°C): 4, or 10, or 40
Contact time (min.): 1, or 5, or 30, or 60
*or client requirements

Interfering substances:
1% milk or 10 g/l yeast extract or 10 g/l sucrose or pH 5 and pH 9 buffer solutions or 5 g/l laurylsulfate
Reduction (lg): ≥ 4,0